Optimization of carbon-nitrogen ratio for production of gibberellic acid by Pseudomonas sp.

In this study, favorable carbon-nitrogen ratio for high yields of gibberellic acid (GA3) production from Pseudomonas sp. was investigated. First of all, optimum carbon (glucose, maltose, sucrose, fructose, lactose) and nitrogen (KNO3, NH4Cl, NaNO3, urea, glycine) sources among the others were chosen. The highest yield of GA3 productivity was found in growth medium supplemented with fructose (168.5 mg/L). NaNO3 was found as a suitable nitrogen source (141 mg/L). Then, in order to determine the optimum carbon-nitrogen ratio, different concentrations of carbon (from 50 mM to 150 mM) and nitrogen (from 17 mM to 47 mM) sources were added in culture media. As a result, optimum carbon-nitrogen ratio for GA3 production from Pseudomonas sp. was found to be 100:17 mM.